|
In dermatopathology, the Tzanck test, also Tzanck smear, is scraping of an ulcer base to look for Tzanck cells. It is sometimes also called the chickenpox skin test and the herpes skin test. Tzanck cells (multinucleated giant cells) are found in: *Herpes simplex *Varicella and herpes zoster *Pemphigus vulgaris *Cytomegalovirus The Tzanck test has been largely replaced by DFA for identification of the herpes virus 〔"The laboratory diagnosis of herpes simplex virus infections." Can J Infect Dis Med Microbiol. 2005 Mar-Apr; 16(2): 92–98. PMCID: PMC2095011〕 ==Procedure== # Unroof vesicle and scrape base w/ sterile surgical blade # Smear onto clean glass slide # Fix w/ gentle heat or air dry # Fix w/ MeOH # Stain w/ Giemsa, methylene blue or Wright’s stain # Microscopic examination using oil immersion lens. (Look for multinucleate giant cells)〔Pettit, Normal Microbiota of the Skin, ATSU School of Osteopathic Medicine Arizona, Lecture Slides. Jan 2013.〕 A modified test can be performed using proprietary agents which requires fewer steps and allows the sample to be fixed quicker. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Tzanck test」の詳細全文を読む スポンサード リンク
|